Information Package / Course Catalogue - Adnan Menderes University

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Enzyme Kinetics

Course Code	: BYK604
Course Type	: Required
Couse Group	: Third Cycle (Doctorate Degree)
Education Language	: Turkish
Work Placement	: N/A

Theory	: 2
Prt.	: 0
Credit	: 2
Lab	: 0
ECTS	: 5

Objectives of the Course

To teach the structure, types, mechanisms of action and inhibition of enzymes that form the basis of life. Quantitative properties of enzyme working mechanisms are explained with numerical examples.

Course Content

Definition and history of enzymes, Substrate specificity regulation of enzymatic activity, allosteric enzymes, chemical kinetics, the Michaelis-Menten equation, kinetic data analysis, enzyme inhibition, factors affecting enzyme activity, two-substrate enzymatic reactions, cofactors and coenzymes, active site-catalytic region, determination and meaning of the Km, Vmax and kcat parameters, examples of applications of enzyme kinetics.

Name of Lecturer(s)

Learning Outcomes

1.	To be able to understand enzyme structures and working principles of enzymes
2.	To be able to identify and evaluate the factors affecting enzyme activity
3.	To be able to determine the catalytic activity of enzymes
4.	Learning enzyme kinetics
5.	To be able to comprehend that enzyme kinetics is a quantitative concept

Recommended or Required Reading

1.	Enzyme Kinetics Irwin H Segel John Willey Sons, INC 1993
2.	Biochemical Calculations Irwin H. Segel John Wiley and sons Newyork,Toronto,Singapore,1982
3.	Harper’s Biochemistry,R.K.Murray,D.K. Granner,P.A.Mayes,V.W.Rodwell. Middle east edition,2000
4.	Lehninger Principles of Biochemistry: International Edition, D.L. Nelson, M.M. Cox, 2017

Weekly Detailed Course Contents

Week 1 - Theoretical

Enzyme structures and working principles of enzymes

Week 2 - Theoretical

Enzyme types and functions of cofactors

Week 3 - Theoretical

Enzyme effect mechanisms and differences between enzymes and chemical catalysts

Week 4 - Theoretical

Flexible enzyme induced fit hypothesis

Week 5 - Theoretical

Factors responsible for catalytic yield of enzymes

Week 6 - Theoretical

Enzyme Kinetics

Week 7 - Theoretical

Enzymatic Activity, Michaelis Menten Equation

Week 8 - Intermediate Exam

Enzyme Kinetics Midterm

Week 9 - Theoretical

Importance and use of Km value

Week 10 - Theoretical

Lineweaver Burk diagram, determination of kinetic values

Week 11 - Theoretical

Reaction Sequences

Week 12 - Theoretical

Calculation of Km values ??using curves

Week 13 - Theoretical

Inhibitors, inhibition types and types of inhibitors

Week 14 - Theoretical

Multi-site and allosteric enzymes

Week 15 - Theoretical

Multi-substrate enzymes and their kinetic mechanisms

Week 16 - Final Exam

Enzyme Kinetics Final Exam

Assessment Methods and Criteria

Type of Assessment	Count	Percent
Midterm Examination	1	%40
Final Examination	1	%60

Workload Calculation

Activities	Count	Preparation	Time	Total Work Load (hours)
Lecture - Theory	14	4	2	84
Midterm Examination	1	18	2	20
Final Examination	1	19	2	21
TOTAL WORKLOAD (hours)				125

Contribution of Learning Outcomes to Programme Outcomes

	PÇ-1	PÇ-2	PÇ-3	PÇ-4	PÇ-5
OÇ-1	5	5	4	5	4
OÇ-2	5	5	4	5	4
OÇ-3	5	4	5	4	5
OÇ-4	5	5	5	4	5
OÇ-5	5	4	5	4	5

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