Information Package / Course Catalogue
Advanced Biochemical Analyses
Course Code: BYK605
Course Type: Required
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 3
Prt.: 0
Credit: 3
Lab: 0
ECTS: 5
Objectives of the Course

Advances in biological sciences largely depend on rapid developments in measurement and monitoring technologies. It is necessary to have knowledge about these new technologies, instrumental methods and basic principles of analytical / industrial processes, in order to reach quality reliable results in selection of suitable devices and methods in researches. The aim of this course is to give the students basic knowledge about advanced techniques in biochemistry and to gain the ability of advanced practical thinking in the analysis of biomolecules.

Course Content

The role of analytical chemistry in biochemistry research, basic analytical principles, classification of quantitative analysis methods, the analysis error calculations, the separation and extraction methods, chromatographic techniques, electrophoretic techniques, the theoretical basis of spectroscopic techniques, UV-VIS spectroscopy, NMR-IR and their use in biochemistry, immunochemical techniques, mass spectroscopy and MALDI-MS technique, examples for applications.

Name of Lecturer(s)
Learning Outcomes
1.Ability to understand modern biochemical techniques and gain research experience
2.To be able to explain the basic principles of modern biochemical instrumental techniques, to understand the operating and application conditions
3.To be able to understand new developments in instrumentation technology
4.Ability to gain perspective in quantitative and qualitative evaluation and purification of biomolecules
5.To be able to understand new diagnostic tools in modern biochemistry laboratories
Recommended or Required Reading
1.Biochemical Methods S sadasivam-a manickam
2.Metod of biochemical analysis. Clarence H. Suelter
Weekly Detailed Course Contents
Week 1 - Theoretical
Basic principles and applications of Hypnated techniques such as LC-MS / MS, CE-MS / MS
Week 2 - Theoretical
Basic principles and applications of advanced mass spectrometric techniques; For example, MALDI-TOF, ESI-TOF, SELDI-TOF etc.
Week 3 - Theoretical
Basic principles and applications of multidimensional techniques; i.e., 2D-PAGE, 2D-DIGE, 2D-LC
Week 4 - Theoretical
Basic principles and applications of stable isotope marking techniques; Ex: ICAT, SILAC, etc.
Week 5 - Theoretical
Basic principles and applications of immunostaining techniques; i.e., ELISA, RIA, etc.
Week 6 - Theoretical
Basic principles and applications of Spectral Techniques; For example; Fluorescence, NMR
Week 7 - Theoretical
Basic principles and applications of Spectral Techniques; For example; CD, FRET, etc.
Week 8 - Intermediate Exam
Advanced Biochemical Analyses Midterm Exam
Week 9 - Theoretical
Discussion and Seminar
Week 10 - Theoretical
Basic principles and applications of X-ray crystallography
Week 11 - Theoretical
Basic principles and applications of imaging techniques; For example; Cyro M, Immuno fluorescence etc.
Week 12 - Theoretical
Basic principles and applications of microarrays; DNA arrays
Week 13 - Theoretical
Basic principles and applications of microarrays; Protein arrays.
Week 14 - Theoretical
Basic principles and applications of microarrays; Glycoarrays.
Week 15 - Theoretical
Comparison and evaluation of techniques; guided problem solving
Week 16 - Final Exam
Advanced Biochemical Analysis Final Exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%40
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory144284
Midterm Examination118220
Final Examination119221
TOTAL WORKLOAD (hours)125
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
OÇ-1
5
5
5
4
5
OÇ-2
5
4
5
4
5
OÇ-3
5
4
5
5
4
OÇ-4
5
5
5
4
5
OÇ-5
5
4
5
5
5
Adnan Menderes University - Information Package / Course Catalogue
2026