Information Package / Course Catalogue - Adnan Menderes University

General Information Unit Staff Academic Information Programme Outcomes Course Structure Diagram Contact Information

Person Responsible for Information Package

Proteomics and Metabolomics: Overview and Significance of Medicine

Course Code	: BYK630
Course Type	: Required
Couse Group	: Third Cycle (Doctorate Degree)
Education Language	: Turkish
Work Placement	: N/A

Theory	: 3
Prt.	: 0
Credit	: 3
Lab	: 0
ECTS	: 3

Objectives of the Course

The aim of this course is to enable students to learn the basic concepts of Proteomics, to have analytical thinking about proteins, proteomes are proteins produced by a cell or organism under certain conditions, how these molecules are related to each other and how they form a biological system and proteomics applications in other sciences.

Course Content

Transcriptional regulation, genetic and protein interactions, tools and analytics, Sample preparation of pure and complex of biological materials, Protein expression and purification. Liquid chromatographic methods and mass spectrometry, 2D electrophoresis

Name of Lecturer(s)

Learning Outcomes

1.	To be able to learn that the proteome is the proteins produced by a cell or organism under certain conditions
2.	To be able to understand how proteins interact and form a biological system
3.	Describe the proteome as sequence, structure, abundance, location, arrangement, interactions and biochemical functions
4.	Learning of protein separation, identification and determination principles
5.	Learning basic information about proteomics database

Recommended or Required Reading

1.	Proteomic and Metabolomic Approaches to Biomarker Discovery:Haleem J Issaq
2.	The Handbook of Metabonomics and Metabolomics:ohn C. Lindon,Jeremy K. Nicholson,Elaine Holmes
3.	Introduction to Proteomics:Daniel C. Lieble

Weekly Detailed Course Contents

Week 1 - Theoretical

Transition from Genomics to Proteomics: Functional genomics at DNA and RNA level; Proteomics' field of activity; Proteomics challenges

Week 2 - Theoretical

General Properties of Proteins: Structure and Function of Proteins

Week 3 - Theoretical

Discrimination techniques used in proteome analysis (I): Principles of two-dimensional gel electrophoresis; Use of two-dimensional gel electrophoresis in proteomics

Week 4 - Theoretical

Discrimination techniques used in proteome analysis (II): Principles of liquid chromatography in proteomics; Multidimensional liquid chromatography

Week 5 - Theoretical

Techniques used for protein identification (I): Protein identification by antibodies; Determination of protein sequences by chemical degradation

Week 6 - Theoretical

Techniques used in protein identification (II): Use of mass spectrometry in proteomics; protein identification using mass spectrometry information

Week 7 - Theoretical

Techniques used for protein determination: Quantitative proteomics with standard two-dimensional gel; quantitative proteomics by mass spectrometry

Week 8 - Intermediate Exam

Proteomics and Metabolomics: Overview and Significance of Medicine Midterm Exam

Week 9 - Theoretical

Proteomics and analysis of protein sequences: Protein families and evolutionary relationships; Basic principles of protein sequence comparison

Week 10 - Theoretical

Structural proteome analysis: The importance of protein structures in proteomics; Techniques for understanding protein structures; Comparison of protein structures

Week 11 - Theoretical

Protein-protein interactions and proteomics: Principles of protein interaction analysis; Protein interaction maps; Proteins and small molecules

Week 12 - Theoretical

The role of protein modifications in proteome analysis: Phosphoproteomics, an overview of protein phosphorylation; Glycoproteomics, the role of glycoproteins in the cell

Week 13 - Theoretical

Protein chips and functional proteome analysis: Different types of protein chips; The emergence of protein chip technology

Week 14 - Theoretical

Proteomic Applications (I): Medical proteomics, disease diagnosis; Pharmaceutical proteomics, drug development

Week 15 - Theoretical

Proteomics Applications (II): Proteomics and plant biotechnology; Proteomics for the analysis of genetically modified plants

Week 16 - Final Exam

Proteomics and Metabolomics: Overview and Significance of Medicine Final Exam

Assessment Methods and Criteria

Type of Assessment	Count	Percent
Midterm Examination	1	%40
Final Examination	1	%60

Workload Calculation

Activities	Count	Preparation	Time	Total Work Load (hours)
Lecture - Theory	14	1	2	42
Midterm Examination	1	14	2	16
Final Examination	1	15	2	17
TOTAL WORKLOAD (hours)				75

Contribution of Learning Outcomes to Programme Outcomes

	PÇ-1	PÇ-2	PÇ-3	PÇ-4	PÇ-5
OÇ-1	5	4	5	4	5
OÇ-2	5	5	4	5	5
OÇ-3	5	4	5	4	5
OÇ-4	5	5	4	5	5
OÇ-5	5	4	4	5	5

Person Responsible for Information Package