Information Package / Course Catalogue
Microbial Biofilms
Course Code: BİO621
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

This course aims to learn the biofilm structures of microorganisms, the qualitative and quantitative methods used in the examination of these structures, to comprehend the mechanisms related to biofilm, to have information about the importance of biofilms in terms of environment and human health, and the prevention of biofilm formation.

Course Content

Microbial biofilm structure and formation mechanism, qualitative and quantitative analysis methods of biofilm formation, methods of preventing biofilm forming microorganisms, importance of biofilm in environment and health

Name of Lecturer(s)
Learning Outcomes
1.To learn the definition of microbial biofilm
2.To learn the groups of microorganisms that cause biofilm development
3.To understand the formation and development mechanisms of biofilms
4.To learn the analysis of biofilm formation by qualitative and quantitative methods
5.To learn the importance of biofilms for the environment and human health
6.Understanding the importance of microbial biofilms in biotechnology
7.To understand the methods applied to prevent biofilm development
8.To learn the effects of biocidal substances on biofilm formation
9.To have knowledge about new environmentally friendly products that can be used in biofilm prevention
Recommended or Required Reading
1.Das S., Kungwani N.A. 2022. Understanding Microbial Biofilms: Fundamentals to Applications. First Edition, Elsevier Science, Amsterdam, Holland, 616p.
2.Abdul B.A.A. 2021. Microbial Biofilms: Properties and Applications in the Environment, Agriculture, and Medicine. First Edition, CRC Press, England, 484p.
3.Ghannoum M., Parsek M., Whiteley M., Mukherjee P.K. 2020. Microbial Biofilms. Second Edition, ASM Press, Washington, USA, 404p.
4.Vickery K. 2020. Microbial Biofilms in Healthcare: Formation, Prevention and Treatment. First Edition, MDPI, Switzerland, 166p.
5.Dincer S., Özdenefe M.S., Arkut A. Bacterial Biofilms. BoD – Books on Demand, Germany, 360p.
6.Seneviratne C.J. 2017. Microbial Biofilms: Omics Biology, Antimicrobials and Clinical Implications. First Edition, CRC Press, England, 290p.
7.Donelli G. 2016. Microbial Biofilms: Methods and Protocols. Second Edition, Springer, New York, USA, 380p.
8.Dhanasekaran D., Thajuddin N. 2016. Microbial Biofilms: Importance and Applications. First Edition, InTech Open, Croatia, 524p.
Weekly Detailed Course Contents
Week 1 - Theoretical
Definition of microbial biofilms, basic concepts and general approach to the subject
Week 1 - Laboratory
Working principles in the laboratory, use of chemical substances and nutrient preparation techniques
Week 2 - Theoretical
Microbial Diversity (Culturable and Non-Culturable Microorganisms
Week 2 - Laboratory
Isolation of microorganisms from various environments (food, soil, moist areas, etc.)
Week 3 - Theoretical
Microbial Biofilm Formation, Growth, Structure and Development Strategy, Physico-Chemical Structure of Adhesion
Week 3 - Laboratory
Isolation of microorganisms from various environments (food, soil, moist areas, etc.)
Week 4 - Theoretical
Genetics of Bacterial Biofilm Quorum Sensing Mechanism
Week 4 - Laboratory
Purification and storage of isolated microorganisms
Week 5 - Theoretical
The Function of Biofilms in the Ecosystem, Effect of Microbial Activity on Surfaces, Bacterial Metabolism in Biofilms
Week 5 - Laboratory
Purification and storage of isolated microorganisms
Week 6 - Theoretical
Molecular Methods for Investigation of Biofilm Communities (PCR, Agarose Gel Electrophoresis, DGGE, FISH)
Week 6 - Laboratory
Analysis of biofilm formation of isolated microorganisms by qualitative method
Week 7 - Theoretical
Biofilm Modeling, Process Analysis and Structural Perspective
Week 7 - Laboratory
Analysis of biofilm formation of isolated microorganisms by qualitative method
Week 8 - Theoretical
Subject Repetition (Midterm)
Week 8 - Laboratory
Analysis of biofilm formation of isolated microorganisms by qualitative method (Midterm)
Week 9 - Theoretical
Biofilm Growth in Biofouling Formation Sampling, Extraction and Molecular Analysis
Week 9 - Laboratory
Application of quantitative method to biofilm forming microorganisms
Week 10 - Theoretical
Microorganisms Attached to Biofilms in Aquatic Systems Indicator and Pathogenic Bacteria
Week 10 - Laboratory
Application of quantitative method to biofilm forming microorganisms
Week 11 - Theoretical
Screening of Biofilm Inhibitors and Inhibitor Analysis
Week 11 - Laboratory
Application of biocides and metals to biofilm-forming microorganisms
Week 12 - Theoretical
Destruction of Biofilm Matrix with Biocides and Metals (Biocides and Metals)
Week 12 - Laboratory
Application of biocides and metals to biofilm-forming microorganisms
Week 13 - Theoretical
Destruction of Biofilm Matrix with Enzymes (Proteases, Glycosidases, Lipases)
Week 13 - Laboratory
Application of enzymes to biofilm-forming microorganisms
Week 14 - Theoretical
Antibiotic resistance of biofilm communities
Week 14 - Laboratory
Application of enzymes to biofilm-forming microorganisms
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory142256
Laboratory143270
Individual Work142370
Midterm Examination1224
Final Examination1224
TOTAL WORKLOAD (hours)204
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
PÇ-14
OÇ-1
4
4
OÇ-2
3
4
4
OÇ-3
4
3
3
4
4
OÇ-4
4
4
4
OÇ-5
4
4
OÇ-6
3
4
OÇ-7
3
3
3
4
OÇ-8
4
4
OÇ-9
4
4
Adnan Menderes University - Information Package / Course Catalogue
2026