Information Package / Course Catalogue
Biotechnological Application in Laboratory Techniques
Course Code: LBT122
Course Type: Area Elective
Couse Group: Short Cycle (Associate's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 3
Objectives of the Course

It is the objective of this course to enable the under graduate students to learn historical development of markers used in past to present time and to learn common types of molecular markers and analysis methods

Course Content

Definition and types of markers. Definition, types and applications of molecular markers. Isolation of nucleic acids (DNA and RNA isolation). RAPD, AFLP, RFLP, Microsatellites (SSR), SNP, EST, Microarray and its analysis methods

Name of Lecturer(s)
Lec. Mürüvvet ABBAK
Learning Outcomes
1.To know about molecular marker types
2.To understand importance of molecular markers in breeding studies
3.Be able to obtain knowledge about applications of molecular markers
4.To understand analyze technique of molecular markers
5.To understand Isolation of nucleic acids (DNA and RNA isolation)
Recommended or Required Reading
1.Temizkan, G., 2013. Moleküler Genetik. Nobel Tip Kitabevi ISBN: 9786053350217
2.Lörz, H., Wenzel, G., 2008. Molecular Marker Systems in Plant Breeding and Crop Improvement (Biotechnology in Agriculture and Forestry) Springer
3.Cox, M.M., Doudna, J., O'Donnell, M., 2012. Molecular biology principle and practice
4.Konuk, M., Moleküler Biyoloji Önemli Notlar. Nobel Yayınları ISBN: 9789755915968.
5.Guimarães EP, Ruane J, Scherf BD, Sonnino A, Dargie JD 2007 Marker-Assisted Selection. Current Status and Future Perspectives in Crops, Livestock, Forestry and Fish.
6.FAO (2011).Molecular genetic characterization of animal genetic resources. FAO Animal Production and Health Guidelines. No: 9, Rome
7.Hawley, R.S. And Walker, M.Y. (2003). Advanced Genetic Analysis. Finding Meaning in a Genome. Blackwell Publishing. Victoria/Australia
Weekly Detailed Course Contents
Week 1 - Theoretical & Practice
General information about biotechnology. History and development of biotechnology.
Week 2 - Theoretical & Practice
Biosystems Microorganisms, Plants and Animals
Week 3 - Theoretical & Practice
Definition and types of Markers (Token)
Week 4 - Theoretical & Practice
Definition, types and uses of molecular markers
Week 5 - Theoretical & Practice
Biotechnology and Application Areas Definition of Biotechnology Applications of Biotechnology in Food, Medicine, Enzyme, Plant, Environment and Genetics
Week 6 - Theoretical & Practice
Chromosome, Gene, Genome etc. basic concepts Nucleic acid Isolation (DNA and RNA isolation)
Week 7 - Theoretical & Practice
Selection criteria of molecular markers
Week 8 - Theoretical & Practice
RAPD (Randomly amplified polymorphic DNA) (Midterm Exam)
Week 9 - Theoretical & Practice
AFLP (Amplified fragment length polymorphism),RFLP (Restriction fragment length polymorphism)
Week 10 - Theoretical & Practice
Microsatellites, Sequencing and EST methods
Week 11 - Theoretical & Practice
SNP (Single point mutations)
Week 12 - Theoretical & Practice
Marker assisted selection
Week 13 - Theoretical & Practice
Uses of markers in plant and animal breeding
Week 14 - Theoretical & Practice
Evaluation of data from molecular markers
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%40
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory140228
Lecture - Practice140228
Midterm Examination1819
Final Examination19110
TOTAL WORKLOAD (hours)75
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
OÇ-1
3
3
5
5
5
4
OÇ-2
3
3
5
5
5
4
OÇ-3
3
3
5
5
5
4
OÇ-4
3
3
5
5
5
4
OÇ-5
3
3
5
5
5
4
Adnan Menderes University - Information Package / Course Catalogue
2026