Information Package / Course Catalogue
Molecular Laboratory Techniques I
Course Code: TBY405
Course Type: Area Elective
Couse Group: First Cycle (Bachelor's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 0
Credit: 3
Lab: 2
ECTS: 4
Objectives of the Course

The aim of this course is to introduce a detailed description of the molecular methods used in biotechnology.

Course Content

The contents of this course include introduction to molecular genetic lab, solution preparation methods, DNA isolation in plants, determination of DNA quantity and quality, PCR, preparation of agarose and polyacrilamide gel, the use of biochemical markers in breeding, hybridization-based molecular techniques and practices used in horticulture breeding, determination of gen expression (cDNA-AFLP, microarray), quantitative determination of gene expression (Real Time PCR), similarity index, the creation and evaluation of the dendograms.

Name of Lecturer(s)
Learning Outcomes
1.Have knowledge about molecular biology laboratory and equipment
2.Have knowledge about methods and techniques used in molecular biology
3.Marker types and analysis are learned
4.Nucleus, chloroplast and mitochondria gene regions are learned
5.Learning bioinformatics programs used in marker technique
Recommended or Required Reading
1.Molecular Markers in Plants Robert J. Henry
Weekly Detailed Course Contents
Week 1 - Theoretical
Introduction to molecular genetic lab, solutions preparation methods
Week 1 - Practice
Introduction to molecular genetic lab, solutions preparation methods
Week 1 - Preparation Work
Reading course-related information from different sources
Week 2 - Theoretical
Week 2 - Practice
Week 2 - Preparation Work
Reading course-related information from different sources
Week 3 - Theoretical
Determination of DNA quantity and quality
Week 3 - Practice
Determination of DNA quantity and quality
Week 3 - Preparation Work
Reading course-related information from different sources
Week 4 - Theoretical
Polymerase chain reaction (PCR)
Week 4 - Practice
Polymerase chain reaction (PCR)
Week 4 - Preparation Work
Reading course-related information from different sources
Week 5 - Theoretical
Preparation of agarose and polyacrilamide gel
Week 5 - Practice
Preparation of agarose and polyacrilamide gel
Week 5 - Preparation Work
Reading course-related information from different sources
Week 6 - Theoretical
The use of biochemical markers in breeding
Week 6 - Practice
The use of biochemical markers in breeding
Week 6 - Preparation Work
Reading course-related information from different sources
Week 7 - Intermediate Exam
Midterm
Week 8 - Theoretical
Hybridization-based molecular techniques and practices used in Horticulture breeding (RFLP)
Week 8 - Practice
Hybridization-based molecular techniques and practices used in Horticulture breeding (RFLP)
Week 8 - Preparation Work
Reading course-related information from different sources
Week 9 - Theoretical
PCR-based molecular breeding techniques and practices used in biotechnology (RAPD, ISSR)
Week 9 - Practice
PCR-based molecular breeding techniques and practices used in biotechnology (RAPD, ISSR)
Week 9 - Preparation Work
Reading course-related information from different sources
Week 10 - Theoretical
PCR-based molecular breeding techniques and practices used in biotechnology (SSR, SRAP)
Week 10 - Practice
PCR-based molecular breeding techniques and practices used in biotechnology (SSR, SRAP)
Week 10 - Preparation Work
Reading course-related information from different sources
Week 11 - Theoretical
PCR-based molecular breeding techniques and practices used in biotechnology (AFLP, CAPs)
Week 11 - Practice
PCR-based molecular breeding techniques and practices used in biotechnology (AFLP, CAPs)
Week 11 - Preparation Work
Reading course-related information from different sources
Week 12 - Theoretical
Determination of gen expression (cDNA-AFLP, microarray)
Week 12 - Practice
Determination of gen expression (cDNA-AFLP, microarray)
Week 12 - Preparation Work
Reading course-related information from different sources
Week 13 - Theoretical
Quantitative determination of gene expression (Real Time PCR)
Week 13 - Practice
Quantitative determination of gene expression (Real Time PCR)
Week 13 - Preparation Work
Reading course-related information from different sources
Week 14 - Theoretical
Similarity index, the creation and evaluation of the dendograms
Week 14 - Practice
Similarity index, the creation and evaluation of the dendograms
Week 14 - Preparation Work
Reading course-related information from different sources
Week 15 - Theoretical
General review
Week 16 - Final Exam
Final exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Attending Lectures1%5
Quiz1%5
Midterm Examination1%30
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory142256
Lecture - Practice3129
Laboratory121124
Midterm Examination1314
Final Examination1314
TOTAL WORKLOAD (hours)97
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
OÇ-1
1
3
4
4
OÇ-2
2
2
OÇ-3
2
4
4
5
3
OÇ-4
3
5
1
1
OÇ-5
1
Adnan Menderes University - Information Package / Course Catalogue
2026