
| Course Code | : TBY405 |
| Course Type | : Area Elective |
| Couse Group | : First Cycle (Bachelor's Degree) |
| Education Language | : Turkish |
| Work Placement | : N/A |
| Theory | : 2 |
| Prt. | : 0 |
| Credit | : 3 |
| Lab | : 2 |
| ECTS | : 4 |
The aim of this course is to introduce a detailed description of the molecular methods used in biotechnology.
The contents of this course include introduction to molecular genetic lab, solution preparation methods, DNA isolation in plants, determination of DNA quantity and quality, PCR, preparation of agarose and polyacrilamide gel, the use of biochemical markers in breeding, hybridization-based molecular techniques and practices used in horticulture breeding, determination of gen expression (cDNA-AFLP, microarray), quantitative determination of gene expression (Real Time PCR), similarity index, the creation and evaluation of the dendograms.
| 1. | Have knowledge about molecular biology laboratory and equipment |
| 2. | Have knowledge about methods and techniques used in molecular biology |
| 3. | Marker types and analysis are learned |
| 4. | Nucleus, chloroplast and mitochondria gene regions are learned |
| 5. | Learning bioinformatics programs used in marker technique |
| 1. | Molecular Markers in Plants Robert J. Henry |
| Type of Assessment | Count | Percent |
|---|---|---|
| Attending Lectures | 1 | %5 |
| Quiz | 1 | %5 |
| Midterm Examination | 1 | %30 |
| Final Examination | 1 | %60 |
| Activities | Count | Preparation | Time | Total Work Load (hours) |
|---|---|---|---|---|
| Lecture - Theory | 14 | 2 | 2 | 56 |
| Lecture - Practice | 3 | 1 | 2 | 9 |
| Laboratory | 12 | 1 | 1 | 24 |
| Midterm Examination | 1 | 3 | 1 | 4 |
| Final Examination | 1 | 3 | 1 | 4 |
| TOTAL WORKLOAD (hours) | 97 | |||
PÇ-1 | PÇ-2 | PÇ-3 | PÇ-4 | PÇ-5 | PÇ-6 | PÇ-7 | PÇ-8 | PÇ-9 | PÇ-10 | PÇ-11 | |
OÇ-1 | 1 | 3 | 4 | 4 | |||||||
OÇ-2 | 2 | 2 | |||||||||
OÇ-3 | 2 | 4 | 4 | 5 | 3 | ||||||
OÇ-4 | 3 | 5 | 1 | 1 | |||||||
OÇ-5 | 1 | ||||||||||