Information Package / Course Catalogue
Applied Molecular Biology
Course Code: TBY303
Course Type: Required
Couse Group: First Cycle (Bachelor's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 0
Credit: 3
Lab: 1
ECTS: 4
Objectives of the Course

The main objective of the Applied Molecular Biology course is to provide knowledge about topics such as cells, organelles, biomolecules, the structure and function of proteins, the structure and function of nucleic acids (DNA and RNA), RNA modifications, gene sequencing, transcriptome analysis, laboratory principles, DNA and RNA isolation, polymerase chain reaction, and electrophoresis methods.

Course Content

Cells, organelles, organisms, Physical and chemical structure of DNA, physical and chemical structure of RNA, Gene structure, genome structure, The structure and function of proteins, In prokaryotes and eukaryotes DNA replication, DNA recombination and repair mechanism, Transcription in prokaryotes, Transcription in eukaryotic, Regulation of transcription in prokaryotes, Regulation of transcription in eukaryotes, RNA modifications, editing and importance of RNA, Application of Polymerase Chain Reactions, gen sequencing.

Name of Lecturer(s)
Learning Outcomes
1.Ability to understand the cell and its structure, and to distinguish the structure and function of DNA and RNA.
2.Ability to understand the structure and function of proteins.
3.Ability to understand DNA and RNA isolation protocols.
4.Understanding the transcription mechanism in eukaryotic and prokaryotic organisms.
5.Ability to understand the techniques used in molecular biology.
Recommended or Required Reading
1.Molecular Biology, Nobel Academic Publishing Consulting Trade. Professor Dr. Mehmet Karataş, 2012
2.Fundamentals of Molecular Genetics, Assoc. Prof. Dr. H. Ümit LÜLEYAP, Nobel Kitapevi, 2008
3.Molecular Biology, Palme Publishing, Prof. Dr. Nihat DİLSİZ, 2009
Weekly Detailed Course Contents
Week 1 - Theoretical
Cells, organelles, organisms
Week 1 - Laboratory
Laboratory rules
Week 2 - Theoretical
Physical and chemical structure of nucleic acids
Week 2 - Laboratory
Introduction of new laboratory equipment
Week 3 - Theoretical
Prokaryotic and Eukaryotic genome structure
Week 3 - Laboratory
Introduction of laboratory chemicals
Week 4 - Theoretical
Structure and functions of proteins
Week 4 - Laboratory
DNA isolation protocols
Week 5 - Theoretical
DNA replication
Week 5 - Laboratory
RNA isolation protocols
Week 6 - Theoretical
DNA repair and repair mechanism
Week 6 - Laboratory
DNA isolation from plants
Week 7 - Theoretical
Prokaryotic transcription
Week 7 - Laboratory
RNA isolation from plants
Week 8 - Theoretical
Transcription in Eukaryotic Organisms (Midterm exam)
Week 9 - Theoretical
Transcriptional regulation in prokaryotes and eukaryotes
Week 9 - Laboratory
DNA and RNA quantifying and spectrophotometry
Week 10 - Theoretical
Electrophoresis methods
Week 10 - Laboratory
Agarose Electrophoresis application
Week 11 - Theoretical
Polymerase Chain Reactions
Week 11 - Laboratory
Application of Polymerase Chain Reactions
Week 12 - Theoretical
RNA modifications, editing and importance of RNA
Week 12 - Laboratory
Application of Polymerase Chain Reactions
Week 13 - Theoretical
Sequencing Techniques
Week 13 - Laboratory
Bioinformatic analysis of sequence results
Week 14 - Theoretical
Transcriptome and Microarrays
Week 14 - Laboratory
Bioinformatic analysis of sequence results
Assessment Methods and Criteria
Type of AssessmentCountPercent
Laboratory1%5
Quiz1%5
Midterm Examination1%30
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory142256
Laboratory141128
Midterm Examination1718
Final Examination1718
TOTAL WORKLOAD (hours)100
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
OÇ-1
3
3
3
2
3
3
3
3
2
1
1
OÇ-2
2
2
2
3
4
2
3
3
1
2
1
OÇ-3
3
3
3
3
3
1
4
2
2
1
1
OÇ-4
3
2
3
2
2
1
2
2
1
1
2
OÇ-5
2
2
1
1
1
1
2
1
2
1
2
Adnan Menderes University - Information Package / Course Catalogue
2026