Information Package / Course Catalogue
Recombinant DNA Technology
Course Code: TBY401
Course Type: Required
Couse Group: First Cycle (Bachelor's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 0
Credit: 2
Lab: 0
ECTS: 3
Objectives of the Course

Aim of this Course is to familiarize students with the most frequently used methods in Recombinant DNA Technology and Genetic Engineering a theoretical point of view

Course Content

Definition of Recombinant DNA and Gene Engineering, General Application Areas, Molecular Methods Used in Recombinant DNA Technology, Polymerase Chain Reaction, Cloning and Expression of Genes, DNA Cutting Enzymes, Cloning and Expression Vectors Used in Gene Transfer, DNA Gel Electrophoresis, Gene Transfer Methods, DNA Sequence Analysis

Name of Lecturer(s)
Learning Outcomes
1.Students learn about the application areas of fundamental analytical techniques used in molecular biology within recombinant DNA technology.
2.They know the historical processes related to the discovery and use of DNA, RNA, and proteins.
3.60 They learn the basic techniques in gene cloning and manipulation.
4.Students learn about the use of recombinant DNA technology in genetic engineering to modify eukaryotic and prokaryotic cells.
5.They learn how to perform recombinant DNA sequencing and use databases.
6.They learn about gene expression and protein production.
7.Students gain knowledge about the practical and biotechnological applications of recombinant DNA technology.
Recommended or Required Reading
1.Principles of Gene Manipulation and Genomics, Seventh Edition, Sandy B., Primrose, Richard M. Twyman, Madlen, MA; Oxford: Blackwell Pub., (2013)
2.Gene Cloning and DNA Analysis An Introduction Fourth Edition T.A Brown, Wiley-Blackwell Pub. (2020)
3.An Introduction to Genetic Engineering, Third Edition, Desmond S. T. Nicholl Cambridge University Press (2008)
4.Moleküler Biyoteknoloji. 2021 Biyoloji Bernard R.glıck, Cheryl L.patten Editör : Teoman Kankılıç. Palme Yayınevi.
Weekly Detailed Course Contents
Week 1 - Theoretical
Introduction and some basic concepts
Week 2 - Theoretical
Definition and application areas of Recombinant DNA Technology and Gene Engineering
Week 3 - Theoretical
Polymerase Chain Reaction
Week 4 - Theoretical
Gel Elecrophoresis of DNA
Week 5 - Theoretical
Restriction enzymes used in gene cloning
Week 6 - Theoretical
Primary vectors used in gene cloning
Week 7 - Theoretical
Ligation and transformation in gene cloning (Midterm exam)
Week 8 - Theoretical
Recombinant DNA transformation techniques
Week 9 - Theoretical
Recombinant DNA selection techniques
Week 10 - Theoretical
DNA sequence analysis
Week 11 - Theoretical
DNA databases
Week 12 - Theoretical
Expression of recombinant genes (vectors)
Week 13 - Theoretical
Expressipon of recombinant genes (induction)
Week 14 - Theoretical
Production stages of recombinant protein
Assessment Methods and Criteria
Type of AssessmentCountPercent
Presentation1%5
Quiz1%5
Midterm Examination1%30
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory132252
Midterm Examination110111
Final Examination111112
TOTAL WORKLOAD (hours)75
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
OÇ-1
5
5
2
3
3
2
3
4
2
3
2
OÇ-2
5
4
2
3
3
2
3
3
2
3
2
OÇ-3
5
5
2
3
3
2
3
4
2
3
2
OÇ-4
5
5
2
3
3
2
3
4
2
3
2
OÇ-5
5
5
3
4
4
2
3
4
2
3
4
OÇ-6
5
5
2
4
3
2
3
4
2
3
5
OÇ-7
5
5
2
4
3
2
3
4
2
3
2
Adnan Menderes University - Information Package / Course Catalogue
2026