Information Package / Course Catalogue
Applied Molecular Biology II
Course Code: TBY304
Course Type: Area Elective
Couse Group: First Cycle (Bachelor's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 0
Credit: 3
Lab: 2
ECTS: 4
Objectives of the Course

The goal of the course is to teach gene expression mechanisms of eukaryotes and prokaryotes.

Course Content

Gene expression in prokaryotes and eukaryotes; transcription, regulatory elements and transcription factors, mRNA processing, genetic code, translation and functional protein synthesis by post-translational modifications

Name of Lecturer(s)
Learning Outcomes
1.At the end of the course students should be able to; know mechanisms of transcription, gene regulation, RNA processing and translation in bacteria & eukaryotes
2.explain how recent genomics and functional genomics advances are altering our views
3.apply molecular knowledge to understand and hypothesize about specific complex systems such as the HIV retrovirus and human disease states with underlying molecular dysfunction
4.interpret the results of experiments using standard molecular techniques such as gel shift, transcription run-on assay, linker scanning promoter analysis, etc
5.explain how classic experiments have led to our current understandings about DNA replication, recombination, transcription
Recommended or Required Reading
1.Moleküler Biyoloji (Protein Sentezi ve Yıkımı). Ed: Ahmet Yıldırım, Fevzi Bardakçı, Mehmet Karataş, Bahattin Tanyolaç. NOBEL Kitabevi
2.Moleküler Biyoloji. Nihat Dilsiz. Palme Yayıncılık.
3.Genes VIII, Benjamin Lewin, New Jersey: Prentice- Hall, 2004
4.Molecular Biology of the Gene, James D. Watson, Tania A. Baker, Stephen P. Bell , Alexander Gann , Michael Levine , Richard Losick, Benjamin Cummings Pub Co., San Francisco, 2003.
5.Agricultural Genetics, 2005, Ahmet OKUMUŞ, OMÜ Faculty of Agriculture lecture notes.Samsun
Weekly Detailed Course Contents
Week 1 - Theoretical
Gene expression in prokaryotes
Week 1 - Laboratory
Introduction to Molecular laboratory
Week 1 - Preparation Work
Preparing presentation from textbooks of and internet
Week 2 - Theoretical
Gene expression in eukaryotes
Week 2 - Laboratory
Molecular markers
Week 2 - Preparation Work
Preparing presentation from textbooks of and internet
Week 3 - Theoretical
Transcription in prokaryotes, its activation and repression and transcription factors
Week 3 - Laboratory
DNA extractions and cloning by PCR
Week 3 - Preparation Work
Preparing presentation from textbooks of and internet
Week 4 - Theoretical
Transcription in prokaryotes, its activation and repression and transcription factors
Week 4 - Laboratory
Running the PCR product on the gel and the process after
Week 4 - Preparation Work
Preparing presentation from textbooks of and internet
Week 5 - Theoretical
Transcription in eukaryotes, its activation and repression and transcription factors
Week 5 - Laboratory
western blot, northern blot Southern Blot analysis
Week 5 - Preparation Work
Preparing presentation from textbooks of and internet
Week 6 - Theoretical
Transcription in eukaryotes, its activation
Week 6 - Laboratory
RNA isolations
Week 6 - Preparation Work
Preparing presentation from textbooks of and internet
Week 7 - Theoretical
RNA processing
Week 7 - Laboratory
Preparation of cDNA library
Week 7 - Preparation Work
Preparing presentation from textbooks of and internet
Week 8 - Theoretical
Preparation of cDNA library (Midterm Exam)
Week 9 - Theoretical
mRNA Stability and Localization
Week 9 - Laboratory
Real-time PCR
Week 9 - Preparation Work
Preparing presentation from textbooks of and internet
Week 10 - Theoretical
DNA methylation
Week 10 - Laboratory
Gradient PCR analysis
Week 10 - Preparation Work
Genomic imprinting
Week 11 - Theoretical
Genetic code
Week 11 - Laboratory
DNA sequence analysis
Week 11 - Preparation Work
Preparing presentation from textbooks of and internet
Week 12 - Theoretical
Next generation DNA sequencing
Week 12 - Laboratory
Next generation DNA sequencing
Week 12 - Preparation Work
Next generation DNA sequencing
Week 13 - Theoretical
Translation in eukaryotes, structure and function of tRNA, ribosomes and regulatory proteins
Week 13 - Laboratory
Western Blot and zymogram
Week 13 - Preparation Work
Preparing presentation from textbooks of and internet
Week 14 - Theoretical
The processing is required form a functional protein after translation
Week 14 - Laboratory
Post-translational modifications
Week 14 - Preparation Work
Preparing presentation from textbooks of and internet
Assessment Methods and Criteria
Type of AssessmentCountPercent
Laboratory1%5
Quiz1%5
Midterm Examination1%30
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory142256
Lecture - Practice42840
Midterm Examination1213
Final Examination1213
TOTAL WORKLOAD (hours)102
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
OÇ-1
1
1
2
3
1
OÇ-2
1
2
2
2
OÇ-3
1
3
2
1
1
OÇ-4
1
2
3
3
3
2
OÇ-5
1
1
Adnan Menderes University - Information Package / Course Catalogue
2026