Information Package / Course Catalogue
Recombinant DNA Technology II
Course Code: TBY402
Course Type: Area Elective
Couse Group: First Cycle (Bachelor's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 0
Credit: 3
Lab: 2
ECTS: 4
Objectives of the Course

Aim of this Course is to familiarize students with the most frequently used methods in Recombinant DNA Technology and Genetic Engineering from both a theoretical and experimental point of view

Course Content

Construction and Use of Genomic and cDNA Libraries, Production of Recombinant Proteins, Production of Vaccines and Hormones in Microorganisms, Transgenic Plants and their Applications, Transgenic Animals and their applications, Impacts of Recombinant DNA and Genetic Engineering

Name of Lecturer(s)
Learning Outcomes
1.At the end of this course students learn basic methods of gene cloning and manipulation for protein expression.
2.At the end of this course students learn how recombinant DNA technology is used in genetic engineering to modify prokaryotic and eukaryotic cells
3.Students gain information about practical and biotechnological applications of recombinant DNA technology
4.They will gain the ability to independently conduct a project consisting of fundamental techniques used in recombinant DNA technology.
5.Students gain the ability to create goal-oriented projects, select and conduct application-oriented laboratory experiments, and interpret the results.
Recommended or Required Reading
1.Principles of Gene Manipulation Sandy B., Primrose, Richard M. Twyman, Madlen, MA; Oxford: Blackwell Pub., (2006)
2.Gene Cloning and DNA Analysis An Introduction Fourth Edition T.A Brown
3.An Introduction to Genetic Engineering (Studies in Biology) (2002) Desmond S. T. Nicholl Cambridge University Press
Weekly Detailed Course Contents
Week 1 - Theoretical
The concepts of living things, the basic components of living things, and genetic material.
Week 2 - Theoretical
Recombinant DNA Technology and Their Applications
Week 2 - Preparation Work
Having knowledge from source books
Week 3 - Theoretical
Construction and Use of Genomic and cDNA Libraries
Week 3 - Preparation Work
Having knowledge from source books
Week 4 - Theoretical
Manipulating DNA in Microorganisms other than E. Coli 1
Week 4 - Preparation Work
Having knowledge from source books
Week 5 - Theoretical
Manipulating DNA in Microorganisms other than E. Coli 2
Week 5 - Preparation Work
Having knowledge from source books
Week 6 - Theoretical
Production of Recombinant Proteins 1
Week 6 - Preparation Work
Having knowledge from source books
Week 7 - Theoretical
Production of Recombinant Proteins 2
Week 7 - Preparation Work
Having knowledge from source books
Week 8 - Theoretical
General review
Week 9 - Theoretical
Production of Vaccines and Hormones in Microorganisms 1
Week 9 - Preparation Work
Having knowledge from source books
Week 10 - Theoretical
Production of Vaccines and Hormones in Microorganisms 2
Week 10 - Preparation Work
Having knowledge from source books
Week 11 - Theoretical
Production of Transgenic Plants
Week 11 - Preparation Work
Having knowledge from source books
Week 12 - Theoretical
Areas of use of Transgenic Plants
Week 12 - Preparation Work
Having knowledge from source books
Week 13 - Theoretical
Impacts of Recombinant DNA and Genetic Engineering on the Biological Sciences, Medicine and Industry
Week 13 - Preparation Work
Having knowledge from source books
Week 14 - Theoretical
Final exam
Week 14 - Preparation Work
Having knowledge from source books
Assessment Methods and Criteria
Type of AssessmentCountPercent
Assignment1%5
Quiz1%5
Midterm Examination1%30
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory151245
Assignment1415
Laboratory151245
Final Examination1415
TOTAL WORKLOAD (hours)100
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
OÇ-1
5
5
4
4
5
5
3
3
3
5
2
OÇ-2
3
5
5
4
4
5
5
3
3
2
2
OÇ-3
4
4
4
5
5
5
3
5
4
4
4
OÇ-4
2
5
5
4
4
4
3
5
2
2
5
OÇ-5
5
5
4
5
4
4
4
4
5
5
5
Adnan Menderes University - Information Package / Course Catalogue
2026