Information Package / Course Catalogue
Advanced Laboratory Techniques
Course Code: VBH531
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 1
Prt.: 2
Credit: 2
Lab: 0
ECTS: 3
Objectives of the Course

The aim of this lecture is to give detailed information about advanced laboratory applications used in food microbiology

Course Content

ELISA (Enzyme Linked Immunosorbent Assay). RIA (Radio Immun Assay). CFT (Complement Fixation). Hemaglutination, hemaglutination inhibition, immunodiffusion (agar-gel precipitation, immunoelectrophoresis, zone electrophoresis), immunohistochemical methods (direkt and indirekt immunfleurosans), molecular techniques,. Immunofleurosans. Examination of foods for Listeria. Examination of milk and dairy products for Brucella. Detection of bacterial toxins in foods

Name of Lecturer(s)
Learning Outcomes
1.To gain sufficient knowledge about advanced laboratory techniques
2.To have sufficient knowledge related with ELISA (Enzyme Linked Immunosorbent Assay). RIA (Radio Immun Assay), CFT (Complement Fixation) molecular techniques
3.To know detailed knowledge about Hemaglutination, hemaglutination inhibition, immunodiffusion (agar-gel precipitation, immunoelectrophoresis, zone electrophoresis),
4.To have sufficient information related with immunohistochemical methods (direct and indirect immunofluorescence), immunofluorescence.
5.To have experience to diagnose Listeria and Brucella in food
6.To gain the ability to use the basic knowledge related with advanced molecular applications in food science.
Recommended or Required Reading
1.Koneman's Color Atlas and Textbook of Diagnostic Microbiology
2.Bergey's manual of systematic bacteriology
3.Handbook of Vertebrate Immunology
4.Veterinary Laboratory Medicine
5.The ELISA Guidebook
6.Temel Mikrobiyoloji
7.İmmunoloji
8.Compendium for foods
Weekly Detailed Course Contents
Week 1 - Theoretical
Introduction to conventional methods in food microbiology
Week 1 - Practice
Presentation of instruments and equipment and medias used in microbiological analysis
Week 2 - Theoretical
Routine applications in conventional microbiological analysis
Week 2 - Practice
Routine practices
Week 3 - Theoretical
Salmonella detection methods in food
Week 3 - Practice
Practice on Salmonella detection methods
Week 4 - Theoretical
Campylobacter detection methods in food
Week 4 - Practice
Practice on Campylobacter detection methods
Week 5 - Theoretical
Listeria detection methods in food
Week 5 - Practice
Practice on Listeria detection methods
Week 6 - Theoretical
Yersinia detection methods in food
Week 6 - Practice
Practice on Yersinia detection methods
Week 7 - Theoretical
Pseudomonas, mold, and yeast detection methods in food
Week 7 - Practice
Practice on Pseudomonas, mold, and yeast detection methods
Week 8 - Intermediate Exam
Midterm exam
Week 9 - Theoretical
Coliform, and E. coli detection methods in food
Week 9 - Practice
Practice on Coliform, and E. coli detection methods
Week 10 - Theoretical
Sulfide reduction bacteria detection methods in food
Week 10 - Practice
Practice on Sulfide reduction bacteria detection methods
Week 11 - Theoretical
ELISA test
Week 11 - Practice
Practice on ELISA test
Week 12 - Theoretical
Polymerase Chain Reaction (PCR)
Week 12 - Practice
Practice on PCR
Week 13 - Theoretical
Multiplex PCR method
Week 13 - Practice
Practice on Multiplex PCR method
Week 14 - Theoretical
Immunomanetic Seperation (IMS)
Week 14 - Practice
Practice on Immunomanetic Seperation (IMS)
Week 15 - Theoretical
Searching for antibiotics
Week 15 - Practice
Practice on searching for antibiotics
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%40
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory140114
Lecture - Practice140228
Midterm Examination19110
Final Examination122123
TOTAL WORKLOAD (hours)75
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
OÇ-1
5
5
5
5
5
4
5
OÇ-2
5
5
5
5
5
5
5
OÇ-3
5
5
5
5
5
5
5
OÇ-4
5
5
5
4
5
5
5
OÇ-5
5
5
5
5
5
5
5
OÇ-6
5
5
5
5
5
5
5
5
Adnan Menderes University - Information Package / Course Catalogue