Information Package / Course Catalogue
Basic Molecular Biological Methods
Course Code: TIB502
Course Type: Required
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 5
Objectives of the Course

Basic molecular bioiogy methods education

Course Content

"Tissue and cell extraction, extraction of cellular components, preparation of in vitro cell culture, RNA and DNA isolation, PCR, genetic diseases, genetic engineering and development, the technique of Southern Blotting, Western and Northern Blotting methods. "

Name of Lecturer(s)
Assoc. Prof. Abdullah YALÇIN
Learning Outcomes
1.Explain the basic learning areas
2.Learning molecular biology concepts
3.Learning molecular biology techniques
4.Acquiring skills for conducting molecular biology techniques
5.Analyze data from molecular biology techniques
Recommended or Required Reading
2.Molecular Cell Biology – Harvey Lodish, Arnold Berk, Chris A. Keiser, Monty Krieger, Anthony Bretscher, Hidde Ploegh, Angelika Amon, Mathew P. Scott - W. H. Freeman; Seventh Edition edition (May 2, 2012)
3.Molecular cloning: A laboratory manual – Michael Green and Joseph Sambrook – Cold Sprıng Harbor Laboratory Press (Fourth edition) 2012
Weekly Detailed Course Contents
Week 1 - Practice
Tissue or cell extraction
Week 2 - Practice
Tissue or cell extraction
Week 3 - Practice
DNA and RNA isolation
Week 4 - Practice
DNA and RNA isolation
Week 5 - Practice
Agarose gel elecrophoresis
Week 6 - Practice
Agarose gel electrophoresis
Week 7 - Practice
Polyacrylamide gel electrophoresis
Week 8 - Intermediate Exam
Midterm exam
Week 9 - Practice
Western Blot
Week 10 - Practice
Western Blot
Week 11 - Practice
Southern Blot
Week 12 - Practice
Southern Blot
Week 13 - Practice
Northern Blot
Week 14 - Practice
Northern Blot
Week 15 - Final Exam
Final Exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%10
Final Examination1%25
Practice13%65
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory131239
Lecture - Practice131239
Midterm Examination120222
Final Examination120222
TOTAL WORKLOAD (hours)122
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
OÇ-1
5
1
1
1
3
OÇ-2
5
1
1
1
3
OÇ-3
2
5
5
2
3
OÇ-4
2
5
5
2
2
OÇ-5
2
5
5
1
3
Adnan Menderes University - Information Package / Course Catalogue