Information Package / Course Catalogue
Techniques in Molecular Biology II
Course Code: BİO548
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

The goal of the course is to teach general molecular biology techniques

Course Content

Several high level molecular biology techniques; gene cloning, RT-PCR, transformation, protein extraction and analysis.

Name of Lecturer(s)
Assoc. Prof. Sare İlknur YAVAŞOĞLU
Learning Outcomes
1.To be able to comprehend recombination
2.To be able to comprehend gene transfer
3.To be able to comprehend gene cloning
4.To be able to comprehend Western blotting technique
5.To be able to comprehend RNA and RT-PCR
Recommended or Required Reading
1.Lecturer notes
2.Sambrook and Russell,(2001) Molecular Clonning, Cold Spring Harbor Laboratory Press, ISBN-0-87969-577-3
3.Temizkan G.,Arda N.(Ed.)(2004),Moleküler Biyolojide Kullanılan Yöntemler,Nobel Kitapevleri,ISBN975-420-347-4
4.Levin B., (2004)Genes VIII, Pearson Education Inc. ISBN-0-19-508956-1
Weekly Detailed Course Contents
Week 1 - Theoretical
Genetic recombination
Week 2 - Theoretical
Gene transfer mechanisms: Conjugation
Week 3 - Theoretical
Gene transfer mechanisms: Transformation
Week 4 - Theoretical
Detection for clones
Week 5 - Theoretical
Producing product from cloned gene
Week 6 - Theoretical
Multipleks PCR
Week 7 - Theoretical
Gen Polimorphisms
Week 9 - Theoretical
Allel spesific PCR
Week 10 - Theoretical
Nested PCR
Week 11 - Theoretical
Protein extraction
Week 12 - Intermediate Exam
Midterm Exam
Week 13 - Theoretical
Protein detection methods
Week 14 - Theoretical
SDS-PAGE
Week 15 - Theoretical
Western Bloting
Week 16 - Theoretical
RNA extraction and RT-PCR
Week 17 - Final Exam
Final Exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%40
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory152260
Lecture - Practice152375
Assignment110212
Midterm Examination120222
Final Examination125227
TOTAL WORKLOAD (hours)196
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
OÇ-1
5
5
5
4
5
5
5
5
5
OÇ-2
5
5
5
4
5
5
5
5
5
OÇ-3
5
5
5
5
5
5
5
5
5
OÇ-4
5
5
5
4
5
5
5
5
5
OÇ-5
5
5
5
4
5
5
5
5
Adnan Menderes University - Information Package / Course Catalogue
2026