Information Package / Course Catalogue
Research Methods of Antimicrobial Materials
Course Code: BİO514
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

This course aims to provide students with information on natural and synthetic antimicrobials and their methods of research which can be used in preventing and protecting microbial contamination of foods, in agriculture, in biological fighting, clinically stopping the development of infectious microorganisms and preventing biofilm formation.

Course Content

Research methods and application areas of synthetic and natural materials which are antibacterial, antimicrobacterial, antiviral, antifungal, antiparasitic

Name of Lecturer(s)
Prof. Esin POYRAZOĞLU
Learning Outcomes
1.To learn antimicrobial substance definition and basic concepts
2.To learn natural antimicrobial substances of animal, plant and microbial origin
3.To learn synthetic antimicrobial substances
4.To learn the mechanisms of action of antimicrobial substances
5.To learn the methods of increasing the action mechanisms of antimicrobial substances
6.To learn the extraction and purification processes of different plant extracts as antimicrobial agents
7.To learn methods of obtaining and purification of bacteriocins from bacteria as antimicrobial agent
8.To learn national microbiology standards (IMS) and European microbiology standards (EUCAST)
9.To learn sensitivity tests of antimicrobial substances
10.To learn the applicability of antimicrobial substances to the industrial environment
Recommended or Required Reading
1.Malcolm Woodbine – 2016. “Antimicrobials and Agriculture”, 8th Edition
2.Dharumadurai Dhanasekaran, Nooruddin Thajuddin, A. Panneerselvam- 2015. “Antimicrobials: Synthetic and Natural Compounds”, 1st Edition.
3.M Taylor – 2014. “Handbook of Natural Antimicrobials for Food Safety and Quality”, 1st Edition.
4.Daryl S. Paulson – 2014. “Topical Antimicrobials Testing and Evaluation”, 2nd Edition.
5.Q. Ashton Acton- 2013. “Antimicrobials—Advances in Research and Application”, 1st Edition.
6.Clinical & Laboratory Standards Institute- 2013. “Performance Standards for Antimicrobial Susceptibility Testing”, Twenty-Third Informational Supplement.
7.Flavia Marinelli, ?Olga Genilloud – 2013. “Antimicrobials: New and Old Molecules in the Fight Against Multi-resistant Bacteria”, 1st Edition.
8.Mahendra Rai – 2011. “Natural Antimicrobials in Food Safety and Quality”, 1st Edition.
9.Arch G. Mainous III, ?Claire Pomeroy – 2010. “Management of Antimicrobials in Infectious Diseases: Impact of Antibiotic Resistance”, 2nd Edition.
10.Richard Schwalbe, ?Lynn Steele-Moore, ?Avery C. Goodwin – 2007. “Antimicrobial Susceptibility Testing Protocols”, 1st Edition.
11.Richard G. Wax, ?Kim Lewis, ?Abigail A. Salyers – 2007. “Bacterial Resistance to Antimicrobials”, 2nd Edition
12.Daryl S. Paulson – 2002. “Handbook of Topical Antimicrobials: Industrial Applications in Consumer”, 2nd Edition.
Weekly Detailed Course Contents
Week 1 - Theoretical
Definition of antimicrobial substance, classification, basic concepts and general approach to subject
Week 1 - Laboratory
Working principles in the laboratory, use of chemicals and media preparation techniques
Week 2 - Theoretical
Properties and applications of antimicrobial substances of animal origin
Week 2 - Laboratory
Extraction and purification of antimicrobial substance from different plant varieties
Week 3 - Theoretical
Properties and applications of antimicrobial substances of plant origin
Week 3 - Laboratory
Extraction and purification of antimicrobial substance from different plant varieties
Week 4 - Theoretical
Properties and applications of antimicrobial substances derived from microorganisms
Week 4 - Laboratory
Obtaining and purifying antimicrobial substances (bacteriocins) from different bacteria
Week 5 - Theoretical
Commonly used antimicrobials and their properties in the food and cosmetic industry
Week 5 - Laboratory
Obtaining and purifying antimicrobial substances (bacteriocins) from different bacteria
Week 6 - Theoretical
Antimicrobial substances and properties widely used in agriculture, textile and medical field
Week 6 - Laboratory
Testing different substances that increase the action mechanisms of antimicrobial substances
Week 7 - Theoretical
Classification, isolation and purification of natural and synthetic antibiotics
Week 7 - Laboratory
Applying the agar disk diffusion method to the obtained antimicrobials
Week 8 - Theoretical
Mechanisms of action of antimicrobial substances (Midterm)
Week 8 - Laboratory
Applying the agar disk diffusion method to the obtained antimicrobials (Midterm)
Week 9 - Theoretical
Mechanisms of action of antimicrobial substances
Week 9 - Laboratory
Applying E test to the antimicrobials obtained
Week 10 - Theoretical
Extraction and purification processes of antimicrobial substances from different plant extracts
Week 10 - Laboratory
Application of the macro dilution method to the obtained antimicrobials
Week 11 - Theoretical
Methods of obtaining bacteriocin from bacteria as natural antimicrobial agent
Week 11 - Laboratory
Application of micro dilution method to the obtained antimicrobials
Week 12 - Theoretical
Antimicrobial susceptibility tests (Diffusion tests-Agar Disc Diffusion Test-E Test)
Week 12 - Laboratory
Application of micro dilution method to the obtained antimicrobials
Week 13 - Theoretical
Dilution Tests-Liquid Micro Dilution-Fluid Macro Dilution Tests
Week 13 - Laboratory
Applying accuracy tests to the obtained antimicrobials
Week 14 - Theoretical
The National Microbiology Standards (IMS) and the European Microbiology Standards (EUCAST)
Week 14 - Laboratory
Comparison and interpretation of results with National and European microbiological standard data
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory143270
Reading142256
Individual Work142370
Midterm Examination1224
Final Examination1224
TOTAL WORKLOAD (hours)204
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
OÇ-1
4
4
4
OÇ-2
4
4
4
OÇ-3
4
4
4
OÇ-4
4
4
4
OÇ-5
4
3
4
4
OÇ-6
4
3
3
4
4
OÇ-7
4
3
4
4
4
OÇ-8
3
4
4
OÇ-9
4
4
3
3
OÇ-10
3
3
3
Adnan Menderes University - Information Package / Course Catalogue
2026