Information Package / Course Catalogue
Methods of Fish Immunology Analysis
Course Code: ZSU538
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 0
Credit: 3
Lab: 2
ECTS: 6
Objectives of the Course

The aim of the course is to give students knowledge about immunological analyzes

Course Content

In this course, students learn about the phagocytosis experiment, respiratory burst experiment, myeloperoxidase test, cortisol analysis in fish, superoxide dismutase analysis, catalase analysis and bacterial challenge experiments.

Name of Lecturer(s)
Learning Outcomes
1.Know the collection, seperation and identification of fish leukocytes.
2.Know the measurement of reactive oxygen species in blood cells
3.Know the measurement of markers of activity in neutrophils
4.Know the measurement of total immunoglobulin and hemolytic complement in serum
5.To be able to use the basic knowledge learned
Recommended or Required Reading
1.Yilmaz S., Balık İmmunolojisi Analiz Yöntemleri, Paradigma Akademi Kitabevi Yayınları, İSTANBUL, 2018
2.Antychowicz J., Kozinska A., 1993. Phagocytosis. In: Siwicki A.K., Anderson D.P. and Waluga J., Eds. Disease Diagnosis and Prevention Methods. FAO-project GCP/INT/JPA. IFI Olsztyn Poland. 89-93
Weekly Detailed Course Contents
Week 1 - Theoretical
Collection, seperation and identification of fish leukocytes
Week 2 - Theoretical
Phagocytosis assay: a microtitter plate
Week 3 - Theoretical
Respiratory burst assay and Potential killing assay
Week 4 - Theoretical
Lysozyme assay
Week 5 - Theoretical
Myeloperoxidase assay
Week 6 - Theoretical
Serum total immunoglobulin assay
Week 7 - Theoretical
Assays of hemolytic complement
Week 8 - Theoretical
Total antiprotease assay
Week 9 - Theoretical
?1-antiprotease assay
Week 10 - Theoretical
Cortisol assay in fish
Week 11 - Theoretical
Superoxide dismutase assay
Week 12 - Theoretical
Catalase assay
Week 13 - Theoretical
Bacterial challenge experiments
Week 14 - Theoretical
Agglutination antibody titer assay
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory141356
Assignment150151
Midterm Examination120121
Final Examination120121
TOTAL WORKLOAD (hours)149
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
OÇ-1
3
3
1
2
3
4
5
OÇ-2
2
3
2
3
2
3
3
OÇ-3
3
4
3
2
3
2
4
OÇ-4
5
2
4
3
4
2
1
OÇ-5
1
2
3
4
5
4
3
Adnan Menderes University - Information Package / Course Catalogue
2026