Information Package / Course Catalogue
Cromatographic Analysis Methods in Pharmacology and Toxicology
Course Code: VFT523
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 1
Prt.: 2
Credit: 2
Lab: 0
ECTS: 4
Objectives of the Course

This course aims to provide information about the chromatographic systems, high-pressure liquid chromatography, gas chromatography and thin layer chromatography to introduce types, components, and operating principles and instruments provide information about the ability to use and apply the information learned to give.

Course Content

Chromatography history, applications, classification, high-pressure liquid krımatografi, gas chromatography, thin layer chromatography, components and principles of operation, method development-validation (validation) and characteristics of the chromatogram.

Name of Lecturer(s)
Learning Outcomes
1.To be informed about chromatographic systems
2.To be informed about working principles of the various chromatographic systems
3.The ability to use and apply the information learned to gain devices.
4.To find out and use resources about the profession in the area.
5.To give lectures and/or presentations and discuss with professionals in the area.
Recommended or Required Reading
1.Veterinary Pharmacology and Therapeutics, 8th Edition, Jim E. Riviere (Editor), Mark G. Papich (Editor), 2009.
2.Modern Pharmacology, 6th Edition, Lippincott Williams and Wilkins, 2004 (Ed. C.R. Craig and R.E. Stitzel)
3.Basic and Clinical Pharmacology, 9th Edition, McGraw-Hill, New York, 2004 (Ed. B. Katzung)
4.Goodman and Gilman’s The Pharmacological Basis of Therapeutics 11th Edition, McGraw-Hill, 2006 (Eds. Brunton, Lazo, Parker, Buxton and Blumenthal)
5.Lippincott's Illustrated Reviews: Pharmacology, 3rd Edition, Lippincott Williams and Wilkins, 2005 (Eds. Howard, Mycek, Harvey &Champe)
6.The Veterinary Formulary edited by Yolande Bishop. London Pharmaceutical Press in association with the British Veterinary Association 2001.
7.Pharmacology. Franklin A. Ahrens. Baltimore, Md. London Williams & Wilkins 1996.
8.The physiological basis of veterinary clinical pharmacology J. Desmond Baggot. Oxford Blackwell Science 2001.
9.Veterinary pharmaclgy and therapeutics edited by H. Richard Adams. Ames, Iwa Iwa State University Press 2001.
Weekly Detailed Course Contents
Week 1 - Theoretical
History and applications of chromatography
Week 1 - Practice
The materials used in pharmacology and toxicology analysis
Week 2 - Theoretical
Types and characteristics of Chromatography
Week 2 - Practice
TLC application
Week 3 - Theoretical
Thin-layer chromatography (TLC) components and principles of operation,
Week 3 - Practice
Parts of the introduction of GK
Week 4 - Theoretical
Gas chromatography (GC) components and principles of operation,
Week 4 - Practice
Introduction to HPLC components
Week 5 - Theoretical
High-pressure liquid chromatography (HPLC) components and principles of operation,
Week 5 - Practice
HPLC-conditioning
Week 6 - Theoretical
High-pressure liquid chromatography (HPLC), working principles,
Week 6 - Practice
HPLC application
Week 7 - Theoretical
High-pressure liquid chromatography (HPLC) varieties-I
Week 7 - Practice
HPLC application
Week 8 - Theoretical
High-pressure liquid chromatography (HPLC) varieties-II (Mid-term exam)
Week 9 - Theoretical
And properties of chromatographic columns
Week 9 - Practice
GK application
Week 10 - Theoretical
HPLC to get ready for the operation and analysis
Week 10 - Practice
GK application
Week 11 - Theoretical
chromatogram
Week 11 - Practice
chromatogram evaluation
Week 12 - Theoretical
Chromatographic analysis method development
Week 12 - Practice
chromatogram evaluation
Week 13 - Theoretical
Chromatographic analysis method validation (validation)
Week 13 - Practice
Liquid-liquid phase extraction
Week 14 - Theoretical
HPLC signs of the fault, the fault detection and troubleshooting
Week 14 - Practice
The solid-liquid phase extraction
Week 15 - Theoretical
Final exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Presentation1%5
Verbal Examination1%5
Midterm Examination1%20
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory142142
Lecture - Practice141242
Midterm Examination1628
Final Examination1134
TOTAL WORKLOAD (hours)96
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
OÇ-1
4
5
5
OÇ-2
4
5
5
5
OÇ-3
4
5
5
5
5
OÇ-4
5
4
5
OÇ-5
5
4
5
5
4
5
Adnan Menderes University - Information Package / Course Catalogue
2026