Information Package / Course Catalogue
| Course Code | : BYF603 |
| Course Type | : Required |
| Couse Group | : Third Cycle (Doctorate Degree) |
| Education Language | : Turkish |
| Work Placement | : N/A |
| Theory | : 2 |
| Prt. | : 2 |
| Credit | : 3 |
| Lab | : 0 |
| ECTS | : 4 |
The course aims to introduce recombinant DNA techniques, RNA/DNA isolation, cDNA synthesis and reverse transcription, electrophoresis methods, PCR and RT-PCR, RFLP methods
Recombinant DNA techniques, RNA/DNA isolation, cDNA synthesis and reverse transcription, electrophoresis methods, PCR and RT-PCR, RFLP methods
| 1. | Learning isolation and extraction techniques |
| 2. | Gaining knowledge on working principle, types and applications of electrophoresis |
| 3. | Learning Southern, Nothern and Western blotting techniques |
| 4. | Learning methods of cDNA synthesis and reverse transcription |
| 5. | Leran about RFLP, PCR and RT-PCR and being able to apply PCR and RT-PCR |
| 1. | Ferit Pehlivan, Biophysics, Hacettepe-Taş Yayınevi, Ankara, 2009 |
| 2. | Şefik Dursun (ed.), Biophysics Lecture Notes, Cerrahpaşa Tıp Fak. Yayınevi, İstanbul, 2010. |
| 3. | Lodish, Berk, Matsudaira ve ark. Molecular cell biology, 5th edition, WH Freeman and Co, NY, 2004. |
| 4. | Old and Primrose, Principles of gene manipulation,5th edition,Blackwell,Oxford,1994 |
| Type of Assessment | Count | Percent |
|---|---|---|
| Presentation | 1 | %2.5 |
| Assignment | 1 | %2.5 |
| Midterm Examination | 1 | %25 |
| Final Examination | 1 | %70 |
| Activities | Count | Preparation | Time | Total Work Load (hours) |
|---|---|---|---|---|
| Lecture - Theory | 13 | 0 | 2 | 26 |
| Lecture - Practice | 13 | 0 | 2 | 26 |
| Assignment | 1 | 8 | 1 | 9 |
| Presentation | 1 | 6 | 1 | 7 |
| Midterm Examination | 1 | 12 | 2 | 14 |
| Final Examination | 1 | 22 | 2 | 24 |
| TOTAL WORKLOAD (hours) | 106 | |||
PÇ-1 | PÇ-2 | PÇ-3 | PÇ-4 | PÇ-5 | PÇ-6 | PÇ-7 | PÇ-8 | PÇ-9 | PÇ-10 | PÇ-11 | PÇ-12 | PÇ-13 | PÇ-14 | PÇ-15 | PÇ-16 | PÇ-17 | PÇ-18 | PÇ-19 | PÇ-20 | |
OÇ-1 | 5 | 5 | 5 | 5 | 5 | 2 | 1 | 4 | 4 | 2 | 2 | 5 | 4 | 1 | 4 | 1 | 1 | 1 | 5 | 2 |
OÇ-2 | 5 | 5 | 5 | 5 | 5 | 1 | 2 | 1 | 1 | 2 | 1 | 5 | 4 | 1 | 4 | 1 | 1 | 1 | 5 | 4 |
OÇ-3 | 5 | 5 | 5 | 5 | 5 | 1 | 1 | 3 | 3 | 2 | 1 | 5 | 3 | 1 | 4 | 1 | 2 | 2 | 5 | 3 |
OÇ-4 | 5 | 5 | 5 | 5 | 5 | 1 | 1 | 3 | 1 | 1 | 1 | 5 | 1 | 1 | 4 | 1 | 1 | 1 | 5 | 1 |
OÇ-5 | 5 | 5 | 5 | 5 | 5 | 1 | 1 | 3 | 1 | 1 | 1 | 5 | 1 | 1 | 4 | 1 | 2 | 4 | 5 | 1 |