Information Package / Course Catalogue
Basic Laboratory Techniques in Genetics
Course Code: ZT446
Course Type: Area Elective
Couse Group: First Cycle (Bachelor's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 0
Credit: 2
Lab: 0
ECTS: 3
Objectives of the Course

The course aims to help students to know molecular genetics laboratory and equipments, and to learn basic principles of extraction, quantitation, preservation and analysing of DNA with molecular genetics techniques and interpreting analysis results.

Course Content

The course content briefly include lab safety, lab equipments and basic lab design, preparation of solutions, sample collection and DNA extraction, quality and quantity measurement of nucleic acids, DNA amplification, cutting DNA with restriction enzymes, gel electrophoresis and imaging, DNA markers, DNA sequencing and bioinformatics.

Name of Lecturer(s)
Prof. İbrahim CEMAL
Learning Outcomes
1.Understands molecular genetics and the importance of laboratory applications in molecular genetics
2.The student becomes familiar with the laboratory and its equipment, and learns the basic principles of laboratory use and safety rules
3.Learning biological sample collection, obtaining high-quality and sufficient nucleic acids, and basic molecular genetic analysis techniques
4.Learning how molecular genetic analysis is used in the identification and breeding of farm animals
5.Learning about the latest developments in molecular genetic analysis methods.
Recommended or Required Reading
6.Concepts of Genetics (8th Edition). Klug, W.S., Cummings, M.R., Spencer, C.A., 2005. Publication Date: March 10, 2005 | ISBN-10: 0131918338 | ISBN-13: 978-0131918337 | Edition: 8th
Weekly Detailed Course Contents
Week 1 - Theoretical
Introduction, presentation of the weekly lesson plan and basic concepts.
Week 2 - Theoretical
Basic rules for laboratory use and fundamentals of laboratory safety.
Week 3 - Theoretical
Molecular genetics laboratory basic equipment, supplies and consumables
Week 4 - Theoretical
Pipette types and pipetting techniques
Week 5 - Theoretical
Electromagnetic spectrum: ultraviolet (UV) and its use in sterilization
Week 6 - Theoretical
The concept of pH and measurement methods
Week 7 - Theoretical
Calculations and Preparation of Laboratory Solutions
Week 8 - Theoretical
Collection of biological samples and nucleic acid isolation
Week 9 - Theoretical
Quality and Quantity Assessment of DNA and RNA
Week 10 - Theoretical
Restriction Enzymes and DNA Cloning via Vectors
Week 11 - Theoretical
DNA Amplification via Polymerase Chain Reaction (PCR)
Week 12 - Theoretical
Electrophoresis techniques, gel imaging and analysis
Week 13 - Theoretical
DNA sequencing, genetic markers
Week 14 - Theoretical
Bioinformatics
Assessment Methods and Criteria
Type of AssessmentCountPercent
Assignment1%10
Quiz1%5
Midterm Examination1%25
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory140235
Assignment1909
Quiz1000
Midterm Examination112113
Final Examination116117
TOTAL WORKLOAD (hours)74
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
OÇ-1
4
3
3
3
3
2
OÇ-2
3
5
3
2
5
4
OÇ-3
5
4
5
5
3
3
OÇ-4
5
5
2
4
4
3
2
2
2
OÇ-5
4
3
4
3
5
2
Adnan Menderes University - Information Package / Course Catalogue
2026