Information Package / Course Catalogue
Advanced Cell and Tissue Culture Techniques
Course Code: TIB602
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 6
Objectives of the Course

Course Content

Name of Lecturer(s)
Learning Outcomes
1.1. Explains primary cultures, short-and long-term cultures
2.2. Explains and performs cultures care, primary cultures, cell line, defining the concepts of continuous cell lines, changing of the media and cell passages, different purposes culture conditions
3.3. Explains characterization of cells in culture, markers of tissue and concepts of transformation
4.Explains cell cloning and selection of specific cell type, purpose and definition of cell cloning, dilution by cloning, cloning in suspension cultures and soft agar method
5.Determines the chromosomal composition of cells. Transformed cell cultures, cell characteristics and transformation studies in culture, transformation genetic and chromosomal abnormalities, studies to determine tumorigenic properties.
Recommended or Required Reading
Weekly Detailed Course Contents
Week 1 - Theoretical
Primary cultures, short-and long-term cultures
Week 2 - Theoretical
Cultures care, primary cultures, cell line,
Week 3 - Theoretical
Defining the concepts of continuous cell lines
Week 4 - Theoretical
Changing of the media and cell passages, different purposes culture conditions
Week 5 - Theoretical
Characterization of cells in culture, markers of tissue and concepts of transformation
Week 6 - Theoretical
Staining, microscopy and photography for the morphological evalution purposes
Week 7 - Theoretical
Cross contaminations and preventing methods
Week 8 - Theoretical
Contamination detection methods, methods for the determination of mycoplasma contamination (Midterm Exam)
Week 9 - Theoretical
Cell cloning and selection of specific cell type, purpose and definition of cell cloning, dilution by cloning
Week 10 - Theoretical
Cloning in suspension cultures and soft agar method
Week 11 - Theoretical
Determination of the chromosomal composition of cells
Week 12 - Theoretical
Transformed cell cultures, cell characteristics and transformation studies in culture
Week 13 - Theoretical
Transformed cell cultures, cell characteristics and transformation studies in culture
Week 14 - Theoretical
Presentation and Discussion
Week 15 - Final Exam
Final Exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Assignment1%5
Verbal Examination1%5
Midterm Examination1%20
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory134165
Lecture - Practice134278
Assignment1101
Midterm Examination1123
Final Examination1224
TOTAL WORKLOAD (hours)151
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
OÇ-1
5
4
3
4
1
OÇ-2
1
4
4
4
2
OÇ-3
1
4
3
4
2
OÇ-4
1
4
4
2
1
OÇ-5
1
4
4
2
1
Adnan Menderes University - Information Package / Course Catalogue
2026