Information Package / Course Catalogue
Molecular Biology Techniques
Course Code: GMP618
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 3
Prt.: 0
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

Gaining knowledge about cell disintegration, separation and purification methods. Learning of DNA and RNA isolation and analysis.

Course Content

Nucleic acids. Methods of cell disintegration. Separation and purification methods (filtration, dialysis, precipitation, centrifugation, etc.). DNA isolation and analysis. RNA isolation and analysis. Duplication of DNA by polymerase chain reaction. Recombinant DNA technology. Methods of nucleic acid hybridization. Protein purification methods.

Name of Lecturer(s)
Assoc. Prof. Olcay BOYACIOĞLU
Learning Outcomes
1.
2.
3.
4.
5.
Recommended or Required Reading
Weekly Detailed Course Contents
Week 1 - Theoretical
Nucleic acids
Week 2 - Theoretical
Cell disintegration methods
Week 3 - Theoretical
Separation and purification methods (filtration, dialysis, precipitation, centrifugation, etc.)
Week 4 - Theoretical
DNA isolation and analysis
Week 5 - Theoretical
DNA isolation and analysis
Week 6 - Theoretical
RNA isolation and analysis
Week 7 - Theoretical
RNA isolation and analysis
Week 8 - Intermediate Exam
Midterm
Week 9 - Theoretical
Duplication of DNA by polymerase chain reaction
Week 10 - Theoretical
Recombinant DNA technology
Week 11 - Theoretical
Recombinant DNA technology
Week 12 - Theoretical
Methods of nucleic acid hybridization
Week 13 - Theoretical
Protein purification methods
Week 14 - Theoretical
Protein purification methods
Week 15 - Theoretical
Student presentation
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%50
Quiz4%10
Attending Lectures1%10
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory142370
Assignment228260
Midterm Examination129130
Final Examination139140
TOTAL WORKLOAD (hours)200
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
OÇ-1
1
1
OÇ-2
2
1
OÇ-3
2
1
OÇ-4
4
3
OÇ-5
2
2
2
Adnan Menderes University - Information Package / Course Catalogue
2026