Information Package / Course Catalogue
| Course Code | : TBY414 |
| Course Type | : Non Departmental Elective |
| Couse Group | : First Cycle (Bachelor's Degree) |
| Education Language | : Turkish |
| Work Placement | : N/A |
| Theory | : 2 |
| Prt. | : 0 |
| Credit | : 3 |
| Lab | : 2 |
| ECTS | : 4 |
The aim of this course is to give the theoretical background of the methods used in protein purification. Isolation of the protein of interest from complex media is vital for the characterization of the function, structure and interactions of proteins or protein groups.
Protein structure and properties, Protein elution, Homogenization, Satrifugation, Gel filtration, Polyacrylamide gel electrophoresis (PAGE), SDS-PAGE, Protein staining in PAGE and SDS-PAGE gels, Isoelectric focusing (IEF), Protein determination
| 1. | Know the principles of homogenization |
| 2. | Knows centrifugation |
| 3. | Knows gel filtration |
| 4. | Know the structure of proteins |
| 5. | Knows PAGE Electrophoresis |
| 1. | Lehninger Principles of Biochemistry, David L. Nelson, Michael M. Cox (2012) |
| 2. | Temel ve İleri Moleküler Biyoloji Yöntemleri Genomik ve Proteomik Analizler, Güler Temizkan, Nazlı Arda, Nobel Tıp Kitabevleri (2018) |
| Type of Assessment | Count | Percent |
|---|---|---|
| Midterm Examination | 1 | %40 |
| Final Examination | 1 | %70 |
| Activities | Count | Preparation | Time | Total Work Load (hours) |
|---|---|---|---|---|
| Lecture - Theory | 13 | 1 | 2 | 39 |
| Assignment | 5 | 2 | 2 | 20 |
| Individual Work | 5 | 2 | 2 | 20 |
| Midterm Examination | 1 | 9 | 1 | 10 |
| Final Examination | 1 | 10 | 1 | 11 |
| TOTAL WORKLOAD (hours) | 100 | |||
PÇ-1 | PÇ-2 | PÇ-3 | PÇ-4 | PÇ-5 | PÇ-6 | PÇ-7 | PÇ-8 | PÇ-9 | PÇ-10 | PÇ-11 | |
OÇ-1 | 4 | 4 | |||||||||
OÇ-2 | 4 | 4 | |||||||||
OÇ-3 | 4 | 4 | |||||||||
OÇ-4 | 4 | 4 | |||||||||
OÇ-5 | 4 | 4 | |||||||||